Standard Operating Procedure (SOP)

Here is a complete Standard Operating Procedure (SOP) for Preparation of Reagent Solution commonly used in analytical laboratories:

1. Purpose

To define a standardized method for the preparation, labeling, storage, and use of reagent solutions used in chemical testing, ensuring accuracy and consistency in analytical procedures.

2. Scope

This SOP applies to all laboratory personnel involved in the preparation of reagent solutions for use in chemical and instrumental analysis at [Company Name].

3. Responsibilities

• Laboratory Analyst:

  • Prepare and label reagents as per the SOP.

  • Maintain proper documentation.

• Lab Supervisor/Manager:

  • Verify and approve the prepared reagent solutions.

  • Ensure availability of required chemicals and glassware.

• Quality Assurance:

  • Periodically review and audit preparation records.

4. Materials and Equipment Required

• Analytical balance (calibrated)

• Measuring cylinders, pipettes, volumetric flasks

• Glass rods/stirrers

• Reagent-grade chemicals

• Deionized/distilled water

• Clean and labeled reagent bottles (preferably amber for light-sensitive reagents)

• PPE (lab coat, gloves, goggles)

5. Safety Precautions

• Use appropriate PPE at all times.

• Handle acids, bases, and volatile reagents inside a fume hood.

• Refer to the Material Safety Data Sheet (MSDS) for each chemical.

• Label all containers with hazard warnings if applicable.

6. Procedure

6.1 General Instructions

1. Clean and dry all glassware before use.

2. Use only reagent-grade chemicals.

3. Use freshly prepared deionized/distilled water.

4. Prepare solutions using accurate measurements (by weight or volume as required).

5. Document all preparations in the Reagent Preparation Logbook.

6.2 Preparation of Reagent Solution

1. Weigh or Measure:

   • Accurately weigh the solid chemical or measure the liquid chemical as required using calibrated equipment.

2. Dissolve and Dilute:

   • Add the chemical to an appropriate volume of deionized/distilled water in a beaker.

   • Stir with a glass rod until fully dissolved.

   • Transfer to a volumetric flask and make up the volume to the mark with water.

3. Transfer and Label:

   • Transfer the solution to a clean, labeled reagent bottle.

   • Label must include:

     • Name of the reagent

     • Concentration

     • Date of preparation

     • Prepared by

     • Expiry date

     • Storage condition

6.3 Labeling Format (Example)

7. Storage and Stability

• Store reagents as per their stability and label instructions (e.g., RT, 2–8°C, light-protected).

• Discard expired or contaminated solutions.

• Maintain a reagent inventory log with status (active/expired).

8. Documentation

• Record details in the Reagent Preparation Logbook, including:

  • Name and concentration of reagent

  • Quantity and batch of chemicals used

  • Date of preparation and expiry

  • Name and signature of preparer and reviewer

9. References

• Laboratory Manual

• Pharmacopeial Methods (e.g., USP, BP, EP)

• ICH Q7: Good Manufacturing Practices

• MSDS of individual chemicals

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