by Dr. Yashashwini Reddy | Aug 11, 2025
Principle of UV-Vis Spectrophotometry The fundamental principle of UV-Vis spectrophotometry is based on the absorption of ultraviolet (190–400 nm) and visible (400–800 nm) light by molecules. When a molecule absorbs light, electrons are promoted from a lower energy...
by Dr. Yashashwini Reddy | Apr 16, 2025
1. What is the basic principle of UV spectroscopy? UV spectroscopy is based on the absorption of ultraviolet (UV) and visible light by molecules. When a molecule absorbs light in the UV-visible range (typically 200-800 nm), electrons in the molecule are excited from a...