Unwanted Peaks in Chromatographic Analysis.

by Dr. Yashashwini Reddy | Apr 29, 2025

Extraneous peaks in chromatographic analysis refer to unwanted or unexpected signals in the chromatogram that do not correspond to the target analytes being measured. These peaks can interfere with the accurate interpretation of the data and can result from various...

What kinds of detectors are available for use in HPLC systems?

by Dr. Yashashwini Reddy | Apr 29, 2025

High-Performance Liquid Chromatography (HPLC) detectors are crucial for identifying and quantifying compounds as they elute from the chromatographic column. Different detectors are chosen based on the nature of the analyte and the sensitivity required. Here’s a...

Developing and Optimizing HPLC Techniques.

by Dr. Yashashwini Reddy | Apr 28, 2025

Developing and optimizing HPLC (High-Performance Liquid Chromatography) techniques is a crucial process in ensuring the accuracy, precision, and reliability of analytical results. This process involves several stages, each of which addresses specific aspects of method...

The Science Behind Gas Chromatography: Principles and Working

by Dr. Yashashwini Reddy | Apr 28, 2025

                   Principle and Working of Gas Chromatography Introduction to Gas Chromatography (GC)A brief overview explaining what GC is and where it is used (such as in chemical analysis, forensic science, environmental testing, etc.). Principle of Gas...

Top 10 Interview questions about HPLC columns.

by Dr. Yashashwini Reddy | Apr 16, 2025

1. What are the different types of HPLC columns? Reversed-phase columns: These are the most commonly used, where the stationary phase is nonpolar (e.g., C18). Polar compounds are retained longer. Normal-phase columns: The stationary phase is polar, while the mobile...