by Dr. Yashashwini Reddy | Aug 8, 2025
1. Principle of HPLC High-Performance Liquid Chromatography (HPLC) works on the principle of separation by differential partitioning of sample components between a mobile phase (liquid) and a stationary phase (solid adsorbent material inside a column). Components with...
by Dr. Yashashwini Reddy | Apr 28, 2025
In High-Performance Liquid Chromatography (HPLC), C8 and C18 columns are two of the most commonly used reversed-phase columns. Their primary difference lies in the length of their carbon chains, which significantly influences their retention behavior, separation...
