by Dr. Yashashwini Reddy | Apr 28, 2025
In High-Performance Liquid Chromatography (HPLC), C8 and C18 columns are two of the most commonly used reversed-phase columns. Their primary difference lies in the length of their carbon chains, which significantly influences their retention behavior, separation...
by Dr. Yashashwini Reddy | Apr 16, 2025
1. What are the different types of HPLC columns? Reversed-phase columns: These are the most commonly used, where the stationary phase is nonpolar (e.g., C18). Polar compounds are retained longer. Normal-phase columns: The stationary phase is polar, while the mobile...
