by Dr. Yashashwini Reddy | Aug 9, 2025
Difference Between C8 and C18 Columns in HPLC Parameter C8 Column C18 Column Full Name Octyl column Octadecyl column Carbon Chain Length 8 carbons 18 carbons Stationary Phase Polarity Less non-polar (moderately hydrophobic) More non-polar (highly hydrophobic)...
by Dr. Yashashwini Reddy | Aug 8, 2025
Steps for HPLC Method Development 1. Define the Purpose Identify the analyte(s) to be separated and quantified. Define method requirements (accuracy, precision, sensitivity, run time). 2. Gather Information Chemical structure, polarity, pKa, solubility of the analyte....
by Dr. Yashashwini Reddy | Aug 8, 2025
1. Principle of HPLC High-Performance Liquid Chromatography (HPLC) works on the principle of separation by differential partitioning of sample components between a mobile phase (liquid) and a stationary phase (solid adsorbent material inside a column). Components with...
by Dr. Yashashwini Reddy | Apr 30, 2025
In pharmaceutical production and testing, a wide range of tools and machinery are used to ensure accurate manufacturing, quality control, and compliance with regulatory standards. These can be broadly categorized into production equipment and testing...
by Dr. Yashashwini Reddy | Apr 29, 2025
System suitability in High-Performance Liquid Chromatography (HPLC) analysis is a set of tests conducted to ensure that the HPLC system is performing optimally for the specific analysis being performed. These checks help determine if the system is capable of producing...
