by Dr. Yashashwini Reddy | Apr 16, 2025
1. What are the different types of HPLC columns? Reversed-phase columns: These are the most commonly used, where the stationary phase is nonpolar (e.g., C18). Polar compounds are retained longer. Normal-phase columns: The stationary phase is polar, while the mobile...
by Dr. Yashashwini Reddy | Apr 16, 2025
1. What is HPLC and why is it used? HPLC (High-Performance Liquid Chromatography) is an analytical technique used to separate, identify, and quantify components in a liquid sample. It’s used in pharmaceuticals, food testing, environmental monitoring, and research due...
by Dr. Yashashwini Reddy | Oct 4, 2024
Introduction to High-Performance Liquid Chromatography (HPLC) High-Performance Liquid Chromatography (HPLC) is a highly efficient and precise analytical technique used to separate, identify, and quantify individual components within a mixture. It has become a key tool...
by Dr. Yashashwini Reddy | Sep 29, 2024
1. What is High-Performance Liquid Chromatography (HPLC)? Answer: High-Performance Liquid Chromatography (HPLC) is a powerful analytical method used to separate, identify, and quantify individual components in a mixture. In the pharmaceutical industry, it plays a...
by Dr. Yashashwini Reddy | Jul 20, 2024
Recovery Factor in Cleaning Validation: Procedure and Importance In the cleaning validation, the recovery factor plays a crucial role in determining how effectively residues can be removed from equipment surfaces. This metric ensures the precision of analytical...
