HPLC Calibration Procedure

by Dr. Yashashwini Reddy | Aug 11, 2025

HPLC Calibration Procedure 1. Introduction High-Performance Liquid Chromatography (HPLC) is a critical analytical technique in pharmaceutical quality control. Regular calibration ensures accuracy, precision, and compliance with regulatory requirements like USP...

Extraneous Peaks in Chromatographic Analysis

by Dr. Yashashwini Reddy | Aug 10, 2025

Extraneous Peaks in Chromatographic Analysis In chromatographic techniques like HPLC and GC, extraneous peaks are unwanted peaks in the chromatogram that are not related to the analyte(s) of interest, mobile phase, or expected impurities. They can distort quantitative...

Different Types of HPLC Detectors

by Dr. Yashashwini Reddy | Aug 9, 2025

Different Types of HPLC Detectors 1. UV/Vis Absorbance Detector Principle: Measures absorbance of analytes at a specific wavelength (Beer-Lambert Law). Types: Fixed Wavelength UV (e.g., 254 nm) Variable Wavelength UV Diode Array Detector (DAD/PDA) – allows spectral...

System Suitability in HPLC Analysis

by Dr. Yashashwini Reddy | Aug 9, 2025

System Suitability in HPLC Analysis What is System Suitability? System Suitability Testing (SST) is a set of analytical checks performed before and during analysis to ensure that the HPLC system and method are capable of producing accurate, precise, and reproducible...

10 Tips for HPLC Analysis In Pharmaceuticals

by Dr. Yashashwini Reddy | Aug 9, 2025

10 Tips for HPLC Analysis in Pharmaceuticals Choose the Right Column Select the correct stationary phase (C18, C8, phenyl, etc.) based on analyte polarity. Consider particle size, column length, and pore size for optimal separation. Prepare Mobile Phase Properly Use...