10 Tips for HPLC Analysis In Pharmaceuticals

by Dr. Yashashwini Reddy | Aug 9, 2025

10 Tips for HPLC Analysis in Pharmaceuticals Choose the Right Column Select the correct stationary phase (C18, C8, phenyl, etc.) based on analyte polarity. Consider particle size, column length, and pore size for optimal separation. Prepare Mobile Phase Properly Use...

Difference Between C8 and C18 Columns in HPLC

by Dr. Yashashwini Reddy | Aug 9, 2025

Difference Between C8 and C18 Columns in HPLC Parameter C8 Column C18 Column Full Name Octyl column Octadecyl column Carbon Chain Length 8 carbons 18 carbons Stationary Phase Polarity Less non-polar (moderately hydrophobic) More non-polar (highly hydrophobic)...

Steps for HPLC Method Development

by Dr. Yashashwini Reddy | Aug 8, 2025

Steps for HPLC Method Development 1. Define the Purpose Identify the analyte(s) to be separated and quantified. Define method requirements (accuracy, precision, sensitivity, run time). 2. Gather Information Chemical structure, polarity, pKa, solubility of the analyte....

Principle of HPLC | HPLC System Working Explained

by Dr. Yashashwini Reddy | Aug 8, 2025

1. Principle of HPLC High-Performance Liquid Chromatography (HPLC) works on the principle of separation by differential partitioning of sample components between a mobile phase (liquid) and a stationary phase (solid adsorbent material inside a column). Components with...

Unwanted Peaks in Chromatographic Analysis.

by Dr. Yashashwini Reddy | Apr 29, 2025

Extraneous peaks in chromatographic analysis refer to unwanted or unexpected signals in the chromatogram that do not correspond to the target analytes being measured. These peaks can interfere with the accurate interpretation of the data and can result from various...