by Dr. Yashashwini Reddy | Apr 17, 2025
1. What is LCMS and how does it work? Explanation: This is a foundational question. LCMS combines liquid chromatography (LC) for separating compounds and mass spectrometry (MS) for detecting and identifying them based on mass-to-charge ratio (m/z). It is widely used...
by Dr. Yashashwini Reddy | Apr 16, 2025
1. What are the different types of HPLC columns? Reversed-phase columns: These are the most commonly used, where the stationary phase is nonpolar (e.g., C18). Polar compounds are retained longer. Normal-phase columns: The stationary phase is polar, while the mobile...
by Dr. Yashashwini Reddy | Apr 16, 2025
What is Gas Chromatography and how does it work? Gas Chromatography (GC) is an analytical technique used to separate and analyze compounds that can be vaporized without decomposition. In GC, a sample is injected into a heated injection port, where it is vaporized. The...
by Dr. Yashashwini Reddy | Apr 16, 2025
1. What is HPLC and why is it used? HPLC (High-Performance Liquid Chromatography) is an analytical technique used to separate, identify, and quantify components in a liquid sample. It’s used in pharmaceuticals, food testing, environmental monitoring, and research due...
by Dr. Yashashwini Reddy | Oct 4, 2024
Principle and Procedure of Column Chromatography Principle of Column Chromatography: Column chromatography is based on the principle of separating compounds based on their relative affinities to a stationary phase and a mobile phase. The stationary phase is usually a...
