by Dr. Yashashwini Reddy | Aug 9, 2025
Difference Between C8 and C18 Columns in HPLC Parameter C8 Column C18 Column Full Name Octyl column Octadecyl column Carbon Chain Length 8 carbons 18 carbons Stationary Phase Polarity Less non-polar (moderately hydrophobic) More non-polar (highly hydrophobic)...
by Dr. Yashashwini Reddy | Apr 28, 2025
In High-Performance Liquid Chromatography (HPLC), C8 and C18 columns are two of the most commonly used reversed-phase columns. Their primary difference lies in the length of their carbon chains, which significantly influences their retention behavior, separation...
