by Dr. Yashashwini Reddy | Aug 11, 2025
Principle of UV-Vis Spectrophotometry The fundamental principle of UV-Vis spectrophotometry is based on the absorption of ultraviolet (190–400 nm) and visible (400–800 nm) light by molecules. When a molecule absorbs light, electrons are promoted from a lower energy...
by Dr. Yashashwini Reddy | May 1, 2025
Principle of Ultraviolet and Visible (UV-Vis) Absorption Spectrophotometry: Ultraviolet and Visible (UV-Vis) absorption spectrophotometry is a technique used to measure the absorption of light by a sample in the ultraviolet and visible regions of the electromagnetic...