by Dr. Yashashwini Reddy | Aug 9, 2025
System Suitability in HPLC Analysis What is System Suitability? System Suitability Testing (SST) is a set of analytical checks performed before and during analysis to ensure that the HPLC system and method are capable of producing accurate, precise, and reproducible...
by Dr. Yashashwini Reddy | Aug 9, 2025
Difference Between C8 and C18 Columns in HPLC Parameter C8 Column C18 Column Full Name Octyl column Octadecyl column Carbon Chain Length 8 carbons 18 carbons Stationary Phase Polarity Less non-polar (moderately hydrophobic) More non-polar (highly hydrophobic)...
by Dr. Yashashwini Reddy | Aug 8, 2025
Why Analytical Method Validation is Required? Analytical Method Validation is essential to ensure that a test method is reliable, reproducible, and suitable for its intended purpose.It is required because: Regulatory Compliance Required by ICH Q2(R1), USFDA, EMA, and...
by Dr. Yashashwini Reddy | Aug 8, 2025
Steps for HPLC Method Development 1. Define the Purpose Identify the analyte(s) to be separated and quantified. Define method requirements (accuracy, precision, sensitivity, run time). 2. Gather Information Chemical structure, polarity, pKa, solubility of the analyte....
by Dr. Yashashwini Reddy | Aug 8, 2025
1. What is Relative Response Factor (RRF)? Definition: The Relative Response Factor is the ratio of the detector response of an analyte to that of a reference standard (usually the active ingredient or an internal standard), under identical chromatographic conditions....
