by Dr. Yashashwini Reddy | Apr 17, 2025
1. What is NMR spectroscopy? NMR spectroscopy is an analytical technique used to determine the structure of organic compounds by analyzing the magnetic properties of atomic nuclei. It provides detailed information about the number, types, and environments of atoms in...
by Dr. Yashashwini Reddy | Apr 17, 2025
1. What is GC-MS and how does it work? Answer: GC-MS combines gas chromatography (GC) for separation of compounds and mass spectrometry (MS) for identification and quantification. The sample is vaporized, separated by GC based on volatility, then ionized in the MS and...
by Dr. Yashashwini Reddy | Apr 17, 2025
1. What is LCMS and how does it work? Explanation: This is a foundational question. LCMS combines liquid chromatography (LC) for separating compounds and mass spectrometry (MS) for detecting and identifying them based on mass-to-charge ratio (m/z). It is widely used...
by Dr. Yashashwini Reddy | Apr 16, 2025
1. What is SOR spectroscopy, and how does it differ from traditional optical rotation methods? SOR (Stress-Optic Rotation) spectroscopy measures changes in the optical rotation of polarized light as it passes through a material under mechanical stress. Unlike...
by Dr. Yashashwini Reddy | Apr 16, 2025
1. What is the basic principle of UV spectroscopy? UV spectroscopy is based on the absorption of ultraviolet (UV) and visible light by molecules. When a molecule absorbs light in the UV-visible range (typically 200-800 nm), electrons in the molecule are excited from a...